Part:BBa_K3113102:Design

P9SN

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Cloning Primers:

fw AATTCGTGAGCGGCTGGCGGCTGTTCAAGAAGATTAGCGGCGGGAGTGCTAGCAGCCCGGAAGATGAAAACCAGAGCCTGGAACAGAAAAACAGCCAGCTGAAACAGGAAATTAGCCAGCTGGAACAGGAAATTCAGCAGCTGGAATATTGAA rev CGCGTTCAATATTCCAGCTGCTGAATTTCCTGTTCCAGCTGGCTAATTTCCTGTTTCAGCTGGCTGTTTTTCTGTTCCAGGCTCTGGTTTTCATCTTCCGGGCTGCTAGCACTCCCGCCGCTAATCTTCTTGAACAGCCGCCAGCCGCTCACG

Source

This sequence was provided by our supervisor PhD student Susanne Pettinger, Westmeyer Lab, Helmholtz Center Munich.

Ljubetič, A., et al. (2017). "Design of coiled-coil protein-origami cages that self-assemble in vitro and in vivo." Nature Biotechnology 35: 1094. https://www.nature.com/articles/nbt.3994#s1, Supplementary Text and Figures